Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Apr 24;168(3930):487-9.
doi: 10.1126/science.168.3930.487.

Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum

P S Carlson

Induction and Isolation of Auxotrophic Mutants in Somatic Cell Cultures of Nicotiana tabacum

P S Carlson. Science. .

Abstract

A method for the selection of auxotrophic mutants from somatic cell cultures of Nicotiana tabacum depends upon the incorporation of 5-bromodeoxyuridine into the DNA of wild-type cells and upon its lack of incorporation into the DNA of auxotrophic cells. There may be more than one functional copy of essential genes in the haploid genome of Nicotiana tabacum.

PubMed Disclaimer

LinkOut - more resources