doi: 10.1016/j.clinbiochem.2007.07.009.
Epub 2007 Aug 10.
Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes
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- PMID: 17854793
- DOI: 10.1016/j.clinbiochem.2007.07.009
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Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes
Clin Biochem.
2007 Nov.
Abstract
Objectives: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping.
Design and methods: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes.
Results: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis.
Conclusions: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2.
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