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. 2007 Nov;40(16-17):1325-7.
doi: 10.1016/j.clinbiochem.2007.07.009. Epub 2007 Aug 10.

Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes

Yingying Gu  1 , Qingge Li
Affiliations

Real-time PCR genotyping of aldehyde dehydrogenase-2 using displacing probes

Yingying Gu et al. Clin Biochem. 2007 Nov.

Abstract

Objectives: This study aimed to develop and validate a rapid, accurate method for aldehyde dehydrogenase-2 gene (ALDH2) genotyping.

Design and methods: We reported a single-tube, real-time PCR method for ALDH2 allele detection using two displacing probes.

Results: The genotyping results of 136 human genomic DNA samples indicated 100% concordance between real-time PCR method and the PCR-RFLP analysis.

Conclusions: The developed method could be used for routine clinical testing and high throughput genetic screening of ALDH2.

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