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. 1991;23(10):1137-42.
doi: 10.1016/0020-711x(91)90155-g.

A new purification procedure for bovine C-reactive protein and serum amyloid P component

M Sarikaputi  1 , M Morimatsu, B Syuto, M Saito, M Naiki
Affiliations

A new purification procedure for bovine C-reactive protein and serum amyloid P component

M Sarikaputi et al. Int J Biochem. 1991.

Abstract

1. A new purification procedure was started with salting-out fractionation of serum proteins at 45-75% saturated ammonium sulfate concentration, followed by HE agarose affinity chromatography by which calcium-dependently bound CRP and SAP were purely eluted with EDTA-containing buffer. 2. Pure CRP and SAP were finally separated by DEAE-5PW HPLC. 3. This procedure gave recovery of 15 and 26%, and fold purification of 2650 and 2400 for CRP and SAP, respectively. 4. Each subunit of CRP and SAP had one intrasubunit disulfide bond, determined by reduction and carboxymethylation.

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