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. 2007 Dec;51(12):4512-4.
doi: 10.1128/AAC.00491-07. Epub 2007 Sep 17.

Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase

Fátima Fonseca  1 , Ana Cristina Sarmento, Isabel Henriques, Bart Samyn, Jozef van Beeumen, Pedro Domingues, Maria Rosário Domingues, Maria José Saavedra, António Correia
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Biochemical Characterization of SFC-1, a class A carbapenem-hydrolyzing beta-lactamase

Fátima Fonseca et al. Antimicrob Agents Chemother. 2007 Dec.

Abstract

The carbapenem-hydrolyzing beta-lactamase SFC-1 from Serratia fonticola UTAD54 was overexpressed in Escherichia coli, purified, and characterized. The enzyme exhibited an apparent molecular mass of 30.5 kDa, determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. SFC-1 hydrolyzes penicillins, cephalosporins, aztreonam, and carbapenems and is inhibited by clavulanic acid, sulbactam, and tazobactam.

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References

    1. Henriques, I., A. Moura, A. Alves, M. J. Saavedra, and A. Correia. 2004. Molecular characterization of a carbapenem-hydrolyzing class A β-lactamase, SFC-1, from Serratia fonticola UTAD54. Antimicrob. Agents Chemother. 48:2321-2324. - PMC - PubMed
    1. Mariotte-Boyer, S., M. H. Nicolas-Chanoine, and R. Labia. 1996. A kinetic study of NMC-A β-lactamase, an Ambler class A carbapenemase also hydrolyzing cephamycins. FEMS Microbiol. Lett. 143:29-33. - PubMed
    1. Péduzzi, J., S. Farzaneh, A. Reynaud, M. Barthélémy, and R. Labia. 1997. Characterization and amino acid sequence analysis of a new oxyimino cephalosporin-hydrolyzing class A β-lactamase from Serratia fonticola CUV. Biochim. Biophys. Acta 1341:58-70. - PubMed
    1. Rasmussen, B. A., K. Bush, D. Keeney, Y. Yang, R. Hare, C. O'Gara, and A. Medeiros. 1996. Characterization of IMI-1 β-lactamase, a class A carbapenem-hydrolyzing enzyme from Enterobacter cloacae. Antimicrob. Agents Chemother. 40:2080-2086. - PMC - PubMed
    1. Saavedra, M. J., L. Peixe, J. C. Sousa, I. Henriques, A. Alves, and A. Correia. 2003. Sfh-I, a subclass B2 metallo-β-lactamase from a Serratia fonticola environmental isolate. Antimicrob. Agents Chemother. 47:2330-2333. - PMC - PubMed

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