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Review
. 2007:380:355-63.
doi: 10.1007/978-1-59745-395-0_22.

Intravital two-photon imaging of T-cell priming and tolerance in the lymph node

Susanna Celli  1 , Philippe Bousso
Affiliations
Review

Intravital two-photon imaging of T-cell priming and tolerance in the lymph node

Susanna Celli et al. Methods Mol Biol. 2007.

Abstract

Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.

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