Time gap between oocyst shedding and antibody responses in mice infected with Cryptosporidium parvum

Abstract

We observed the time gap between oocyst shedding and antibody responses in mice (3-week-old C57BL/6J females) infected with Cryptosporidium parvum. Oocyst shedding was verified by modified acid-fast staining. The individually collected mouse sera were assessed for C. parvum IgM and IgG antibodies by enzyme-linked immunosorbent assay from 5 to 25 weeks after infection. The results showed that C. parvum oocysts were shed from day 5 to 51 post-infection (PI). The IgM antibody titers to C. parvum peaked at week 5 PI, whereas the IgG antibody titers achieved maximum levels at week 25 PI. The results revealed that IgM responses to C. parvum infection occurred during the early stage of infection and overlapped with the oocyst shedding period, whereas IgG responses occurred during the late stage and was not correlated with oocyst shedding. Hence, IgM antibody detection may prove helpful for the diagnosis of acute cryptosporidiosis, and IgG antibody detection may prove effective for the detection of past infection and endemicity.

Fig. 1

C. parvum oocysts in mice feces detected by modified acid fast staining. Bar, 10 um.

Fig. 2

Number of oocysts per gram of feces counted after modified acid fast staining from the C57BL mice after C. parvum infection.

Fig. 3

Serum antibody titer of C. parvum-infected C57BL mice detected via ELISA.