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. 2007 Sep;45(3):229-32.
doi: 10.3347/kjp.2007.45.3.229.

Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis

Pyo-Yun Cho  1 , Tae-Im Kim, Shunyu Li, Sung-Jong Hong, Min-Ho Choi, Sung-Tae Hong, Yong -e Chung
Affiliations

Metacercarial proteins interacting with WD40-repeat protein of Clonorchis sinensis

Pyo-Yun Cho et al. Korean J Parasitol. 2007 Sep.

Abstract

The WD40-repeat proteins serve as a platform coordinating partner proteins and are involved in a range of regulatory cellular functions. A WD40-repeat protein (CsWD1) of Clonorchis sinensis previously cloned is expressed stage-specifically in the tegumental syncytium of C. sinensis metacercariae. In the present study, interacting proteins with the CsWD1 protein was purified by immunoprecipitation and 2 dimension gel electrophoresis from the C. sinensis metacercaria soluble extract, and tryptic peptides were analyzed by LC/ESI-MS. Putative partner proteins were annotated to be actin-2, glyceraldehyde-3-phosphate dehydrogenase, and hypothetical and unmanned proteins. The CsWD1 protein was predicted to contain 3 conserved actin-interacting residues on its functional surface. With these results, the CsWD1 protein is suggested to be an actin-interacting protein of C. sinensis.

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Figures

Fig. 1
Fig. 1
Purification of partner proteins of the CsWD1 protein. (A) Putative interacting proteins in a 2-dimensional gel. Arrow indicates the native CsWD1 protein which was proven by peptide sequencing as described in the text. Circle locates putative partner protein. (B) Control group. Spots of upper and lower rows are heavy and light chains of immunoglobulin G, respectively.
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References

    1. Allwood EG, Anthony RG, Smertenko AP, Reichelt S, Drobak BK, Doonan JH, Weeds AG, Hussey PJ. Regulation of the pollen-specific actin-depolymerizing factor LIADF1. Plant Cell. 2002;14:2915–2927. - PMC - PubMed
    1. Cho PY, Kim TI, Yoo WG, Li S, Hong SJ, Kim TY, Park YS, Song KY, Choi MH, Hong ST, Chung YJ, LoVerde PT, Osman A. Molecular cloning and characterization of WD40-repeat protein from Clonorchis sinensis. Parasitol Res. 2007 (in press, DOI 10.1007/s00436-007-0721-6) - PubMed
    1. Ida K, Yaha I. Cooperation of two actin-binding proteins, cofilin and Aip1, in Saccharomyces cerevisiae. Genes Cells. 1999;4:21–32. - PubMed
    1. Kim TI, Cho PY, Li S, Hong ST, Choi MH, Hong SJ. Partner proteins that interact with Clonorchis sinensis WD40-repeat protein. Parasitol Res. 2007 (in press, DOI 10.1007/s00436-007-0625-5) - PubMed
    1. Mohri K, Ono S. Actin filament disassembling activity of Caenorhabditis elegans actin-interacting protein 1 (UNC-78) is dependent on filament binding by a specific ADF/cofilin isoform. J Cell Sic. 2003;166:4107–4118. - PubMed

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