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. 2007 Oct 21;13(39):5261-6.
doi: 10.3748/wjg.v13.i39.5261.

Expression of periostin and its clinicopathological relevance in gastric cancer

Jun-Sheng Li  1 , Guang-Wen Sun, Xiao-Ying Wei, Wen-Hao Tang
Affiliations

Expression of periostin and its clinicopathological relevance in gastric cancer

Jun-Sheng Li et al. World J Gastroenterol. .

Abstract

Aim: To investigate the expression and localization of periostin in gastric cancer and its clinical relevance.

Methods: Reverse transcriptase polymerase chain reaction was used to measure periostin mRNA expression. Western blotting was carried out to detect periostin protein expression. Immunohistochemistry was performed to localize and quantify the expression of periostin in benign gastric diseases and gastric cancer, and immunostaining results were correlated with gastric cancer pathological stages.

Results: Periostin expression was low at both mRNA and protein levels in normal gastric tissues, but was overexpressed in gastric cancer tissues. Immunohistochemical staining revealed that periostin was overexpressed in primary gastric cancer, as well as in metastatic lymph nodes, but only faint staining was found in benign gastric ulcers. By quantitative analysis of the immunostaining results, periostin expression was increased 2.5-4-fold in gastric cancer, compared to that in benign gastric disease, and there was a trend toward increasing periostin expression with tumor stage.

Conclusion: This observation demonstrated that periostin was overexpressed in gastric cancer and lymph node metastasis, which suggests that periostin plays an important role in the progression and metastasis of gastric cancer.

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Figures

Figure 1
Figure 1
RT-PCR analysis of periostin mRNA expression in human gastric cancer (T) and normal gastric tissues (N). Periostin was differentially expressed in gastric cancer tissues (T) compared with normal gastric tissues (N) from the same patient. The expression of β-actin was used as an internal control.
Figure 2
Figure 2
Periostin protein expression in normal and cancer tissue samples. Tissue extracts from normal or gastric cancer tissue samples were subjected to immunoblot analysis with a polyclonal antiperiostin antibody. The results shown in the T and N lanes are for cancer and normal tissues, respectively, from which RT-PCR was performed (A). Over fivefold more periostin was detected in gastric cancer tissues than in normal tissues from the same patient (B).
Figure 3
Figure 3
Immunohistochemical analysis of periostin expression in normal gastric tissues, benign gastric ulcers, gastric cancer tissues, as well as lymphoid metastasis from gastric cancer. The tissue sections were immunostained with a polyclonal antibody. The positive staining for periostin protein is shown with a brown color. All sections were counterstained with hematoxylin showing a blue color. (A) Negative control; (B) benign gastric ulcer; (C) stageIgastric cancer; (D) stageIIgastric cancer; (E) stage III gastric cancer; (F) stage IV gastric cancer; (G) lymph node metastasis. The average MOD of periostin staining from stageI-IV gastric cancer was significantly higher than that from normal gastric tissues in each group (H) (P < 0.05).
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