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Case Reports
. 2007 Nov;45(11):3840-3.
doi: 10.1128/JCM.01041-07. Epub 2007 Sep 19.

"Mycobacterium ulcerans subsp. shinshuense" isolated from a skin ulcer lesion: identification based on 16S rRNA gene sequencing

Kazue Nakanaga  1 , Norihisa Ishii, Koichi Suzuki, Kazunari Tanigawa, Masamichi Goto, Tsutomu Okabe, Hideaki Imada, Akemi Kodama, Tomotada Iwamoto, Hiroshi Takahashi, Hajime Saito
Affiliations
Case Reports

"Mycobacterium ulcerans subsp. shinshuense" isolated from a skin ulcer lesion: identification based on 16S rRNA gene sequencing

Kazue Nakanaga et al. J Clin Microbiol. 2007 Nov.

Abstract

We describe the fourth reported case involving "Mycobacterium ulcerans subsp. shinshuense." Compared to previous cases, the infection was more invasive with extensive ulceration from the elbow to the forearm. Definitive identification involved IS2404 detection, 16S rRNA gene sequencing, and analysis of the 16S rRNA gene 3'-terminal region and the virulence plasmid pMUM001.

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Figures

FIG. 1.
FIG. 1.
PCR targeting IS2404 specific for M. ulcerans. (a) Lane M, 100-bp ladder marker; lane 1, DNA sample extracted from paraffin-embedded skin (patient); lane 2, M. ulcerans 97-107 (African strain); lane 3, M. ulcerans 5143 (Mexican strain), lane 4; M. ulcerans 1615 (Malaysian strain); lane 5, negative control. (b) Lanes 1 to 8, DNA samples extracted from each of eight serially sectioned skin specimens taken on 4 April; lane 9, DNA sample extracted from a fresh skin biopsy specimen taken on 28 March; lane 10, negative control; lane M, 100-bp ladder marker.
FIG. 2.
FIG. 2.
PCR for the presence of the genes on pMUM001. DNA samples were extracted from M. ulcerans 97-107 (African strain) (a), “M. ulcerans subsp. shinshuense” 753 (b), and purified AFB from a skin specimen taken on 4 April (c). Lanes M, 100-bp ladder markers; lanes 1, repA (413 bp); lanes 2, parA (501 bp); lanes 3, serine/threonine protein kinase gene MUP011 (479 bp); lanes 4, loading domain of mls (560 bp); lanes 5, acyltransferase domain of mls (504 bp); lanes 6, rep type II thioesterase gene (500 bp); lanes 7, rep type III ketosynthase gene (496 bp);lanes 8, rep P450 hydroxylase gene (500 bp).
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