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. 2007 Oct;71(1):87-9.
doi: 10.1016/j.mimet.2007.08.002. Epub 2007 Aug 15.

Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides

Shadi Riazi  1 , Sara Dover, Yevgeniy Turovskiy, Michael L Chikindas
Affiliations

Commercial ampholytes used for isoelectric focusing may interfere with bioactivity based purification of antimicrobial peptides

Shadi Riazi et al. J Microbiol Methods. 2007 Oct.

Abstract

BioRad's Rotofor system has been frequently used for the purification of proteins and smaller peptides such as bacteriocins. In this study, we report that some commercially available ampholytes used with the Rotofor isoelectric focusing system possess antimicrobial activity, which may interfere with the purification of bacteriocins and bacteriocin-like substances.

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Figures

Fig. 1
Fig. 1
Antimicrobial activity distribution of Bio-Lyte® (A), Ampholine™ (B), and Pharmalyte™ (C) ampholytes after preparative isoelectric focusing. The inhibitory activity was determined by well diffusion assay using M. luteus ATCC 10420 as the indicator strain. Figure inserts iA and iiA show inhibition zones caused by Bio-Lyte® and nisin (10 mg/ml), respectively, in well diffusion assay against M. luteus. Similarly figure inserts iB and iC are visual representation of zones of inhibition caused by Ampholine™ and Pharmalyte™, respectively, after being fractioned through Rotofor®.
Fig. 1
Fig. 1
Antimicrobial activity distribution of Bio-Lyte® (A), Ampholine™ (B), and Pharmalyte™ (C) ampholytes after preparative isoelectric focusing. The inhibitory activity was determined by well diffusion assay using M. luteus ATCC 10420 as the indicator strain. Figure inserts iA and iiA show inhibition zones caused by Bio-Lyte® and nisin (10 mg/ml), respectively, in well diffusion assay against M. luteus. Similarly figure inserts iB and iC are visual representation of zones of inhibition caused by Ampholine™ and Pharmalyte™, respectively, after being fractioned through Rotofor®.
Fig. 1
Fig. 1
Antimicrobial activity distribution of Bio-Lyte® (A), Ampholine™ (B), and Pharmalyte™ (C) ampholytes after preparative isoelectric focusing. The inhibitory activity was determined by well diffusion assay using M. luteus ATCC 10420 as the indicator strain. Figure inserts iA and iiA show inhibition zones caused by Bio-Lyte® and nisin (10 mg/ml), respectively, in well diffusion assay against M. luteus. Similarly figure inserts iB and iC are visual representation of zones of inhibition caused by Ampholine™ and Pharmalyte™, respectively, after being fractioned through Rotofor®.
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