Argentinean Lactococcus lactis bacteriophages: genetic characterization and adsorption studies

Abstract

Aims: Characterization of four virulent Lactococcus lactis phages (CHD, QF9, QF12 and QP4) isolated from whey samples obtained from Argentinean cheese plants.

Methods and results: Phages were characterized by means of electron microscopy, host range and DNA studies. The influence of Ca(2+), physiological cell state, pH and temperature on cell adsorption was also investigated. The double-stranded DNA genomes of these lactococcal phages showed distinctive restriction patterns. Using a multiplex PCR, phage QP4 was classified as a member of the P335 polythetic species while the three others belong to the 936 group. Ca(2+) was not needed for phage adsorption but indispensable to complete cell lysis by phage QF9. The lactococci phages adsorbed normally between pH 5 and pH 8, and from 0 degrees C to 40 degrees C, with the exception of phage QF12 which had an adsorption rate significantly lower at pH 8 and 0 degrees C.

Conclusions: Lactococcal phages from Argentina belong to the same predominant groups of phages found in other countries and they have the same general characteristics.

Significance and impact of the study: This work is the first study to characterize Argentinean L. lactis bacteriophages.