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. 2007 Oct;43(2):140-5.
doi: 10.1016/j.ymeth.2007.04.004.

Detection of microRNAs by Northern blot analyses using LNA probes

Eva Várallyay  1 , József Burgyán, Zoltán Havelda
Affiliations

Detection of microRNAs by Northern blot analyses using LNA probes

Eva Várallyay et al. Methods. 2007 Oct.

Abstract

MicroRNAs (miRNAs) are small, endogenous, non-coding RNA species, about 21 nucleotides in length, which modulate the expression of animal and plant target genes at the post-transcriptional level. It has been shown that miRNA based gene regulation plays a pivotal role in pathways involved in growth and development. Understanding miRNA mediated processes requires new technologies enabling efficient detection of small RNA species. Here we report the optimization of a miRNA Northern blot detection method based on LNA (locked nucleic acid)-modified oligonucleotide hybridization. This technique allows sensitive and highly specific detection of mature miRNAs.

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