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Review
. 2007 Sep 25:3:35.
doi: 10.1186/1746-160X-3-35.

A pigmented calcifying cystic odontogenic tumor associated with compound odontoma: a case report and review of literature

Phuu P Han  1 , Hitoshi Nagatsuka, Chong H Siar, Hidetsugu Tsujigiwa, Mehmet Gunduz, Ryo Tamamura, Silvia S Borkosky, Naoki Katase, Noriyuki Nagai
Affiliations
Review

A pigmented calcifying cystic odontogenic tumor associated with compound odontoma: a case report and review of literature

Phuu P Han et al. Head Face Med. .

Abstract

Background: Pigmented intraosseous odontogenic lesions are rare with only 47 reported cases in the English literature. Among them, pigmented calcifying cystic odontogenic tumor, formerly known as calcifying odontogenic cyst, is the most common lesion with 20 reported cases.

Methods: A case of pigmented calcifying cystic odontogenic tumor associated with odontoma occurring at the mandibular canine-premolar region of a young Japanese boy is presented with radiographic, and histological findings. Special staining, electron microscopic study and immunohistochemical staining were also done to characterize the pigmentation.

Results: The pigments in the lesion were confirmed to be melanin by Masson-Fontana staining and by transmission electron microscopy. The presence of dendritic melanocytes within the lesion was also demonstrated by S-100 immunostaining.

Conclusion: The present case report of pigmented calcifying cystic odontogenic tumor associated with odontoma features a comprehensive study on melanin and melanocytes, including histochemical, immunohistochemical and transmission electron microscopic findings.

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Figures

Figure 1
Figure 1
Panoramic radiograph demonstrated a well-defined radiolucent lesion with sclerotic border in left mandibular canine region (white arrowhead). Small, radio-opaque masses with comparable radio densities to that of the surrounding teeth were seen inside the radiolucent lesion.
Figure 2
Figure 2
The epithelial lining (at the thickest portion) showed basal palisaded cuboidal cells and suprabasal stellate cells. Small focal calcifications within the epithelium can also be observed. (H&E, 20×). One of the small organoid denticles is shown in the inset.
Figure 3
Figure 3
Odontogenic epithelium and ghost cells stained with Masson-Fontana for melanin pigmentation. Melanin pigments were detected within the epithelial cells, in the ghost cells and also lying freely within the extracellular connective tissue. (Masson Fontana, 40×).
Figure 4
Figure 4
Transmission electron micrograph of the cyst lining epithelium. Melanosomes were seen within the epithelial cells. A pseudoglandular space lined by basement membrane was marked by asterisk (4,200×).
Figure 5
Figure 5
Irregular melanosomes with some inclusions could be observed with higher magnification under transmission electron microscope (24,000×).
Figure 6
Figure 6
The presence of S-100 positive cells with cellular processes within the ghost cells aggregates (S100 immunostaining with AEC chromogen, 40×).
See this image and copyright information in PMC

References

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