Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2008 Mar;12(3):555-60.
doi: 10.1007/s11605-007-0303-z. Epub 2007 Sep 29.

Helicobacter genotyping and detection in peroperative lavage fluid in patients with perforated peptic ulcer

N A P Komen  1 , M J O E Bertleff, L J van Doorn, J F Lange, P W de Graaf
Affiliations

Helicobacter genotyping and detection in peroperative lavage fluid in patients with perforated peptic ulcer

N A P Komen et al. J Gastrointest Surg. 2008 Mar.

Abstract

Introduction and objectives: Certain Helicobacter pylori genotypes are associated with peptic ulcer disease; however, little is known about associations between the H. pylori genotype and perforated peptic ulcer (PPU). The primary aim of this study was to evaluate which genotypes are present in patients with PPU and which genotype is dominant in this population. The secondary aim was to study the possibility of determining the H. pylori status in a way other than by biopsy.

Materials and methods: Serum samples, gastric tissue biopsies, lavage fluid, and fluid from the nasogastric tube were collected from patients operated upon for PPU. By means of PCR, DEIA, and LIPA the presence of the "cytotoxin associated gene" (cagA) and the genotype of the "vacuolating cytotoxin gene" were determined.

Results: Fluid from the nasogastric tube was obtained from 25 patients, lavage fluid from 26 patients, serum samples from 20 patients and biopsies from 18 patients. Several genotypes were found, of which the vacA s1 cagA positive strains were predominant. Additionally, a correlation was found between the H. pylori presence in biopsy and its presence in lavage fluid (p=0.015), rendering the latter as an alternative for biopsy. Sensitivity and specificity of lavage fluid analysis were 100% and 67%, respectively.

Conclusion: This study shows the vacA s1 cagA positive strain is predominant in a PPU population. The correlation found between the H. pylori presence in biopsy and its presence in lavage fluid suggests that analysis of the lavage fluid is sufficient to determine the H. pylori presence. Risks associated with biopsy taking may be avoided.

PubMed Disclaimer

References

    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1136/gut.50.4.460', 'is_inner': False, 'url': 'https://doi.org/10.1136/gut.50.4.460'}, {'type': 'PMC', 'value': 'PMC1773187', 'is_inner': False, 'url': 'https://pmc.ncbi.nlm.nih.gov/articles/PMC1773187/'}, {'type': 'PubMed', 'value': '11889062', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/11889062/'}]}
    2. Higham J, Kang JY, Majeed A. Recent trends in admissions and mortality due to peptic ulcer in England: Increasing frequency of haemorrhage among older subjects. Gut 2002;50:460–464. - PMC - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1046/j.1523-5378.2003.00139.x', 'is_inner': False, 'url': 'https://doi.org/10.1046/j.1523-5378.2003.00139.x'}, {'type': 'PubMed', 'value': '12752726', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/12752726/'}]}
    2. Gisbert JP, Pajares JM. Helicobacter pylori infection and perforated peptic ulcer prevalence of the infection and role of antimicrobial treatment. Helicobacter 2003;8:159–167. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1016/j.amjsurg.2005.07.019', 'is_inner': False, 'url': 'https://doi.org/10.1016/j.amjsurg.2005.07.019'}, {'type': 'PubMed', 'value': '16226957', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/16226957/'}]}
    2. Sarosi GA Jr, Jaiswal KR, Nwariaku FE, Asolati M, Fleming JB, Anthony T. Surgical therapy of peptic ulcers in the 21st century: More common than you think. Am J Surg 2005;190:775–779. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'PubMed', 'value': '1728121', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/1728121/'}]}
    2. Holcombe C, Omotara BA, Eldridge J, Jones DM. H. pylori, the most common bacterial infection in Africa: A random serological study. Am J Gastroenterol 1992;87:28–30. - PubMed
    1. {'text': '', 'ref_index': 1, 'ids': [{'type': 'DOI', 'value': '10.1586/14737159.1.3.290', 'is_inner': False, 'url': 'https://doi.org/10.1586/14737159.1.3.290'}, {'type': 'PubMed', 'value': '11901834', 'is_inner': True, 'url': 'https://pubmed.ncbi.nlm.nih.gov/11901834/'}]}
    2. van Doorn LJ. Detection of Helicobacter pylori virulence-associated genes. Exp Rev Mol Diagn 2001;1:290–298. - PubMed

Substances