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. 1976 May;73(5):1513-7.
doi: 10.1073/pnas.73.5.1513.

Isolation and propagation of a segment of the simian virus 40 genome containing the origin of DNA replication

T E Shenk, P Berg

Isolation and propagation of a segment of the simian virus 40 genome containing the origin of DNA replication

T E Shenk et al. Proc Natl Acad Sci U S A. 1976 May.

Abstract

Heteroduplex DNA molecules formed from two DNAs that differ from each other by a deletion can be cleaved at the mismatched region (a deletion loop) with the single-strand-specific S1 endonuclease. A heteroduplex DNA molecule, constructed from the DNA of simian virus 40 (SV40) mutant with a deletion of the map region 0.54-0.55 and the DNA of a second SV40 mutant having a deletion of the map segment 0.70-0.73, is cleaved twice with S1 endonuclease. One of the products is a DNA fragment of about 0.13 the length of SV40 DNA which contains the origin of SV40 DNA replication (0.67 on the SV40 DNA map).

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References

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