Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Jul 19;12(7):1455-70.
doi: 10.3390/12071455.

The anti-apoptosis effects of daidzein in the brain of D-galactose treated mice

Zhen Mao  1 , Yuan-lin Zheng, Yan-qiu Zhang, Bao-ping Han, Xiao-wan Zhu, Qing Chang, Xiang-bin Hu
Affiliations

The anti-apoptosis effects of daidzein in the brain of D-galactose treated mice

Zhen Mao et al. Molecules. .

Abstract

The purpose of this study was to explore the neuroprotective effects of daidzein on the apoptotic pathway in the hippocampus and cortex of D-galactose treated mice. For this purpose we have examined the expression of bcl-2 mRNA, bax mRNA and caspase-3 in the hippocampus and cortex of D-galactose-treated mice after fed with 10 or 5 mg/kg of daidzein. The results of in situ hybridization experiments indicate that daidzein could help increase the transcriptions of bcl-2 and decrease the transcriptions of bax in those brain regions of D-galactose-treated mice. Furthermore, immunohistochemical studies showed that daidzein could reduce the expression of caspase-3 in both brain regions. These results suggest that daidzein in soybean can inhibit the D-gal induced apoptosis via Bcl-2/Bax apoptotic pathway and be a potential medical candidate for neurodegeneration therapy.

PubMed Disclaimer

Figures

Figure 1
Figure 1
The structures of 17β-estradiol and daidzein.
Figure 2
Figure 2
Effects of daidzein on the expression levels of bcl-2 mRNA assessed by non-radioactive in situ hybridization in D-gal-treated mice hippocampus. (A) Aging control. (B) Vehicle control. (C) Da-10 group. (D) Da-5 group.
Figure 3
Figure 3
Effects of daidzein on the expression levels of bcl-2 mRNA assessed by non-radioactive in situ hybridization in D-gal-treated mice cortexes. (A) Aging control. (B) Vehicle control. (C) Da-10 group. (D) Da-5 group.
Figure 4
Figure 4
Effects of daidzein on the expression levels of bax mRNA assessed by non-radioactive in situ hybridization in D-gal-treated mice hippocampus. (A) Aging control. (B) Vehicle control. (C) Da-10 group. (D) Da-5 group.
Figure 5
Figure 5
Effects of daidzein on the expression levels of bax mRNA assessed by non-radioactive in situ hybridization in D-gal-treated mice cortexes. (A) Aging control. (B) Vehicle control. (C) Da-10 group. (D) Da-5 group.
Figure 6
Figure 6
Effects of daidzein on the expression levels of caspase-3 in D-gal-treated mice hippocampus. (A) Aging control. (B) Vehicle control. (C) Da-10 group. (D) Da-5 group.
Figure 7
Figure 7
Effects of daidzein on the expression levels of caspase-3 in D-gal-treated mice cortexes. (A) Aging control. (B) Vehicle control. (C) Da-10 group. (D) Da-5 group.
See this image and copyright information in PMC

References

    1. Rice-Evans C. A., Miller N. J., Paganga G. Structure-antioxidant Activity Relationships of Flavonoids and Phenolic Acids. Free Radical Bio. Med. 1996;20:933–956. - PubMed
    1. Schreihofer D. A. Transcriptional regulation by phytoestrogens in neuronal cell lines. Mol. Cell. Endocrinol. 2005;231:13–22. - PubMed
    1. Kuiper G. G. J. M., Lemmen J. G., Carlsson B., Corton J. C., Safe S. H., van der Saag P. T., van der Burg B., Gustafsson J.-A. Interaction of Estrogenic Chemicals and Phytoestrogens with Estrogen Receptor β. Endocrinology. 1998;139:4252–4263. - PubMed
    1. Lephart E. D., West T. W., Weber K. S., Rhees R. W., Setchell K. D. R., Adlercreutz H., Lund T.. D. Neurobehavioral effects of dietary soy phytoestrogens. Neurotoxicol. Teratol. 2002;24:5–16. - PubMed
    1. File S. E., Hartley D. E., Alom N., Rattray M. Soya phytoestrogens change cortical and hippocampal expression of BDNF mRNA in male rats. Neurosci. Lett. 2003;338:135–138. doi: 10.1016/S0304-3940(02)01391-5. - DOI - PubMed

Publication types

MeSH terms