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. 1976 May;73(5):1749-53.
doi: 10.1073/pnas.73.5.1749.

Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells

M Shoyab, M N Dastoor, M A Baluda

Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells

M Shoyab et al. Proc Natl Acad Sci U S A. 1976 May.

Abstract

The integration site of avian myeloblastosis virus (AMV) proviral DNA in DNA from leukemia chicken myeloblasts has been studied by three sequential nucleic acid hybridizations that can localize the proviral DNA according to the repetitiveness of the adjacent cellular DNA regions. First, large denatured cellular DNA fragments (2.1 x 10(6) daltons) were reassociated and fractionated according to sequence reiteration frequenct. Next, DNA remaining single-stranded in each fraction was immobilized on nitrocellulose filters hybridized with an excess of unlabeled 70S RNA from Rous-associated virus-0 to saturate the endogenous proviral DNA sequences.

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References

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