Investigating translation initiation using Drosophila molecular genetics

Abstract

Genetic tools enable insights into how translation controls development of a multicellular organism. Different genetic approaches offer the ability to manipulate the Drosophila genome in very precise ways, thereby allowing the investigation of how translation factors work in the context of a whole organism. We present here an overview of selected techniques used to identify genes involved in translation initiation, and quantitative methods to characterize phenotypes caused by mutations in genes encoding translation initiation or regulatory factors.