doi: 10.1111/j.1365-2958.1991.tb02099.x.
Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome
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- PMID: 1791763
- DOI: 10.1111/j.1365-2958.1991.tb02099.x
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Physical and genetic map of the Neisseria gonorrhoeae strain MS11-N198 chromosome
Mol Microbiol.
1991 Oct.
Abstract
A macro-restriction map of the Neisseria gonorrhoeae chromosome was constructed using the enzymes Nhel and Spel. Combinations of one- and two-dimensional electrophoresis of completely or partially digested chromosomal DNA were performed to align the restriction fragments. The chromosome is circular, with an estimated size of 2.33 Mb +/- 35 kb. A genetic map was derived from the physical map; positions of over 60 defined loci were determined by Southern hybridization.
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