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. 2007 Oct 31;129(43):12946-7.
doi: 10.1021/ja076222f. Epub 2007 Oct 5.

Product deuterium isotope effect for orotidine 5'-monophosphate decarboxylase: evidence for the existence of a short-lived carbanion intermediate

Krisztina Toth  1 , Tina L Amyes, Bryant M Wood, Kui Chan, John A Gerlt, John P Richard
Affiliations

Product deuterium isotope effect for orotidine 5'-monophosphate decarboxylase: evidence for the existence of a short-lived carbanion intermediate

Krisztina Toth et al. J Am Chem Soc. .
No abstract available

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Figures

Scheme 1
Scheme 1
Scheme 2
Scheme 2
Figure 1
Figure 1
Partial 1H NMR spectrum (500 MHz) of UMP from decarboxylation of OMP (2 mM) catalyzed by OMPDC from S. cerevisiae (24 nM) in 50/50 (v/v) H2O/D2O at pL 7.3 and 25 °C. Key: (▼) Doublet due to the C-6 proton of [6-1H]-UMP; (•) Doublets (not resolved) due to the anomeric protons of [6-1H]-UMP and [6-2H]-UMP; (×) Doublet due to the C-5 proton of [6-1H]-UMP; formula image Singlet due to the C-5 proton of [6-2H]-UMP.
Scheme 3
Scheme 3
See this image and copyright information in PMC

References

    1. Residues are numbered according to the sequence for the enzyme from yeast.

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