Review
doi: 10.1016/0163-7258(91)90063-r.
Significance and measurement of DNA double strand breaks in mammalian cells
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- PMID: 1792238
- DOI: 10.1016/0163-7258(91)90063-r
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Review
Significance and measurement of DNA double strand breaks in mammalian cells
Pharmacol Ther.
1991.
Abstract
Techniques for measuring DNA double strand breaks in mammalian cells are being used increasingly by researchers studying both physiological processes, such as recombination, replication, and apoptosis, as well as pathological processes, such as clastogenesis induced by ionizing radiation, chemotherapeutic drugs, and chemical toxicants. In this review we evaluate commonly used assays for measuring DNA double strand breaks, focusing on neutral filter elution and pulsed field gel electrophoresis, and explore the advantages and limitations of applying these techniques to problems of current interest in carcinogenesis and genetic toxicology.
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