Vascular endothelial growth factor and nitric oxide production in response to hypoxia in the choroid plexus in neonatal brain
- PMID: 17924979
- PMCID: PMC8095499
- DOI: 10.1111/j.1750-3639.2007.00104.x
Vascular endothelial growth factor and nitric oxide production in response to hypoxia in the choroid plexus in neonatal brain
Abstract
Damage to the choroid plexus in 1-day-old Wistar rats subjected to hypoxia was investigated. The mRNA and protein expression of hypoxia-inducible factor-1alpha (HIF-1alpha), endothelial, neuronal, inducible nitric oxide synthase (eNOS, nNOS, iNOS), and vascular endothelial growth factor (VEGF) along with nitric oxide (NO) production and VEGF concentration was up-regulated significantly in hypoxic rats. Ultrastructurally, the choroid plexus epithelial cells showed massive accumulation of glycogen. A striking feature was the extrusion of cytoplasmic fragments from the apical cell surfaces into the ventricular lumen following the hypoxic insult. Intraventricular macrophages showed increased expression of complement type 3 receptors, major histocompatibility complex class I and II antigens, and ED1 antigens. Following an intravenous injection of horseradish peroxidase (HRP), a large number of intraventricular macrophages were labeled suggesting enhanced leakage of the tracer from the blood vessels in the choroid plexus connective tissue stroma into the ventricular lumen. We suggest that increased production of NO in hypoxia is linked to the structural alteration of the choroid plexus, and along with VEGF, may lead to increased vascular permeability. Melatonin treatment reduced VEGF and NO levels as well as leakage of HRP suggesting its potential value in ameliorating damage in choroid plexus pathologies.
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References
-
- Alonso M, Collado PS, Gonzalez‐Gallego J (2006) Melatonin inhibits the expression of the inducible isoform of nitric oxide synthase and nuclear factor kappa B activation in rat skeletal muscle. J Pineal Res 41:8–14. - PubMed
-
- Banker BQ, Larroche JC (1962) Periventricular leukomalacia of infancy. A form of neonatal anoxic encephalopathy. Arch Neurol 7:386–410. - PubMed
-
- Black SM, Bedolli MA, Martinez S, Bristow JD, Ferriero DM, Soifer SJ (1995) Expression of neuronal nitric oxide synthase corresponds to regions of selective vulnerability to hypoxia‐ischaemia in the developing rat brain. Neurobiol Dis 2:145–155. - PubMed
-
- Bolanos JP, Almeida A (1999) Roles of nitric oxide in brain hypoxia‐ischemia. Biochim Biophys Acta 411:415–436. - PubMed
-
- Breier G, Albrecht U, Sterrer S, Risau W (1992) Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation. Development 114:521–532. - PubMed
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