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. 2007 Dec;34(12):807-11.
doi: 10.1007/s10295-007-0257-x. Epub 2007 Oct 10.

A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates

Tina Lütke-Eversloh  1 , Gregory Stephanopoulos
Affiliations

A semi-quantitative high-throughput screening method for microbial L-tyrosine production in microtiter plates

Tina Lütke-Eversloh et al. J Ind Microbiol Biotechnol. 2007 Dec.

Abstract

In this study, we developed a fluorescence-based assay for quantifying the aromatic amino acid L-tyrosine in small sample volumes employing 96-well microtiter plates. The method was based on the specific derivatisation of L-tyrosine with 1-nitroso-2-naphthol and the formation of a stable complex for the fluorescent determination of L-tyrosine concentrations. The original procedure for L-tyrosine measurements in blood or tissue samples (Waalkes and Udenfriend in J Lab Clin Med 50:733-736, 1957) was modified to a simple assay suitable for high-throughput screening of L-tyrosine producing microorganisms such as engineered Escherichia coli.

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