[Screening human gastric carcinoma-associated antigens by serologic proteome analysis]

Abstract

Background & objective: It is urgent to find the specific or relatively specific biomarkers in the study of gastric carcinoma. This study was to screen human gastric carcinoma-associated antigens by serologic proteome analysis (SERPA).

Methods: Total protein were isolated from 10 specimens of human gastric carcinoma by 2-dimensional electrophoresis (2-DE). The immunoblotting imaging films were obtained and the differential reacting protein spots were recognized. Then the differential reacting proteins were obtained in the replica gel by matching analysis, and identified by peptide mass fingerprint (PMF) based on matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and database searching.

Results: Fourteen differentially expressed proteins (react only to the patient's serum) were found. Of the 14 proteins, 13 were identified as human gastric carcinoma-associated antigens, including heterogeneous nuclear ribonucleoprotein K (hnRNP K), hnRNP H1, hnRNP H2, cytokeratin 18 (CK18), crystal structure of Fkbp52 C-terminal domain complex with the C-terminal peptide meevd of hot-shock protein 90 (Hsp90), serum vitamin D-binding protein precursor (DBP), selenium-binding protein 1 (SBP1), eukaryotic translation elongation factor 1 delta (eEF-1), ACTB protein, brain creatine kinase (CKB), proteasome 26S ATPase subunit 3 (PSMC3), dimethylarginine dimethylaminohydrolase 1(DDAH-1), and NADH dehydrogenase Fe-S protein 1.

Conclusions: Thirteen human gastric carcinoma-associated antigens were found by SERPA. The results provide foundation for screening molecular biomarkers for the diagnosis, therapy, prognostic evaluation, and prevention of human gastric carcinoma.