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. 2008 Jan;18(1):169-74.
doi: 10.1007/s10895-007-0260-7. Epub 2007 Oct 11.

Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol

Flávia Rodrigues de Oliveira Silva  1 , Ricardo Elgul Samad, Laércio Gomes, Lilia Coronato Courrol
Affiliations

Enhancement of europium emission band of europium tetracycline complex in the presence of cholesterol

Flávia Rodrigues de Oliveira Silva et al. J Fluoresc. 2008 Jan.

Abstract

We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H(2)O(2), the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in a simple method to measure the cholesterol quantity in a solution. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems.

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