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. 2007 Dec;73(23):7740-3.
doi: 10.1128/AEM.01079-07. Epub 2007 Oct 12.

TiO2 photocatalysis causes DNA damage via fenton reaction-generated hydroxyl radicals during the recovery period

Gaëtan Gogniat  1 , Sam Dukan
Affiliations

TiO2 photocatalysis causes DNA damage via fenton reaction-generated hydroxyl radicals during the recovery period

Gaëtan Gogniat et al. Appl Environ Microbiol. 2007 Dec.

Abstract

Here, we show that resistance of Escherichia coli to TiO2 photocatalysis involves defenses against reactive oxygen species. Results support the idea that TiO2 photocatalysis generates damage which later becomes deleterious during recovery. We found this to be partly due to DNA attack via hydroxyl radicals generated by the Fenton reaction during recovery.

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Figures

FIG. 1.
FIG. 1.
Survival ratios of wild-type E. coli MG1655 (×) and rpoS::Tn10 (▪), dps::kan (formula image), katE::Tn10 katG::Tn10 (▴), and katE::Tn10 katG::Tn10 dps::kan (Δ) mutants with 60 min of illumination with active TiO2 (Degussa P25, 1 g/liter). Data points indicate the mean values and standard deviations of three or more independent experiments.
FIG. 2.
FIG. 2.
Sensitivities of wild-type E. coli MG1655 (×) and Δfur::kan (□), katE::Tn10 katG::Tn10 (▴), and ΔsodA sodB::MudIIPR3 (★) mutants to TiO2 photocatalysis (Degussa P25, 1 g/liter). Data points indicate the mean values and standard deviations of three or more independent experiments.
FIG. 3.
FIG. 3.
Sensitivities of wild-type E. coli MG1655 (×) and dps::kan (formula image), ΔrecA srl::Tn10 (▾), and dps::kan ΔrecA srl::Tn10 (○) mutants to (A) TiO2 photocatalysis (Degussa P25, 1 g/liter) and (B) light plus inactive TiO2 (Huntsman, 1 g/liter). Data points indicate the mean values and standard deviations of three or more independent experiments.
FIG. 4.
FIG. 4.
Sensitivities after incubation either with (dashed lines) or without (plain lines) 20,000 U catalase. Sensitivities are shown for (A) E. coli MG1655 (×) and katE::Tn10 katG::Tn10 (▴) and katE::Tn10 katG::Tn10 dps::kan (Δ) mutants, (B) Δfur::kan (□) and rpoS::Tn10 (▪) mutants, and (C) dps::kan (formula image) and dps::kan ΔrecA srl::Tn10 (○) mutants to TiO2 photocatalysis (Degussa P25, 1 g/liter) or, (D) with inactive TiO2 (Huntsman, 1 g/liter), the dps::kan ΔrecA srl::Tn10 mutant (○), the dps::kan mutant with catalase (formula image), and the dps::kan ΔrecA srl::Tn10 mutant with catalase (★). Data points indicate the mean values and standard deviations of three or more independent experiments.
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