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. 2007 Nov;4(11):903-5.
doi: 10.1038/nmeth1111. Epub 2007 Oct 14.

Direct selection of human genomic loci by microarray hybridization

Thomas J Albert  1 , Michael N Molla, Donna M Muzny, Lynne Nazareth, David Wheeler, Xingzhi Song, Todd A Richmond, Chris M Middle, Matthew J Rodesch, Charles J Packard, George M Weinstock, Richard A Gibbs
Affiliations

Direct selection of human genomic loci by microarray hybridization

Thomas J Albert et al. Nat Methods. 2007 Nov.

Abstract

We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.

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