The UGT2B17 gene deletion polymorphism and risk of prostate cancer. A case-control study in Caucasians

Abstract

Background: UDP-glucuronosyltransferase (UGT) 2B17 is a phase II metabolizing enzyme that mediates the glucuronidation of C(19) steroids. A deletion polymorphism in the UGT2B17 gene is associated with a substantial reduction in glucuronidation activity in vitro.

Methods: We examined the association between the UGT2B17 deletion polymorphism and the risk of incident prostate cancer in a population-based study from central Arkansas that included 411 Caucasian cases and 397 Caucasian controls. We developed a novel high-throughput procedure that uses real-time PCR and allelic discrimination for genotyping analysis.

Results: The prevalence of the UGT2B17 deletion [(0/0)] was 12% in the controls, which was consistent with previous population estimates and with Hardy Weinberg equilibrium. There was no association between the UGT2B17 deletion polymorphism and prostate cancer risk in unconditional logistic regression analysis. Compared to the wild-type group (+/+), the adjusted odds ratio (OR) was 0.89 (95% CI=0.55-1.45) for the homozygous deletion (0/0), and the OR was 0.99 (95% CI=0.73-1.35) for the heterozygote group (+/0).

Conclusion: These findings show that the UGT2B17 deletion polymorphism is not associated with prostate cancer risk in Caucasians.

Figure 1

Gene structure and primer and probe locations for the UGT2B17 multiplex real-time PCR assay. The roman numerals I–VI indicate the exons of the UGT2B17 gene on the wild-type allele. The deletion allele is shown below the wild-type allele indicating the 120 kb that are deleted, including the entire UGT2B17 gene. Red arrows indicate the primers that will amplify the deletion allele and the red bar (with fluorescent label Joe) indicates the deletion probe. Blue arrows indicate the primers that will amplify exon 1 of UGT2B17 from the wild-type allele and the blue bar (with fluorescent label Fam) indicates the deletion probe.