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. 1991 Nov-Dec;82(6):503-5.
doi: 10.1093/oxfordjournals.jhered.a111138.

Linkage of the chicken MHC to the nucleolus organizer region visualized using non-isotopic in situ hybridization

M Dominguez-Steglich  1 , C Auffray, M Schmid
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Linkage of the chicken MHC to the nucleolus organizer region visualized using non-isotopic in situ hybridization

M Dominguez-Steglich et al. J Hered. 1991 Nov-Dec.

Abstract

Linkage of the chicken major histocompatibility complex (B-complex) to the nucleolus organizer regions (NOR) has been confirmed in situ. Nonradioactive hybridization to metaphase chromosomes using a pool of biotinylated cDNA probes for the B-F, B-L beta, and B-G genes of the B-complex gave positive signals on one or a pair of microchromosomes. Subsequent staining with silver nitrate proved these microchromosomes to be the NOR-bearing pair. The value of employing nonisotopic in situ hybridization techniques for mapping of genes and gene complexes, especially when dealing with microchromosomes, is stressed.

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