doi: 10.1016/0005-2736(76)90203-0.
Localization of enzymes in specialized regions of the microsomal membrane
- PMID: 179597
- DOI: 10.1016/0005-2736(76)90203-0
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Localization of enzymes in specialized regions of the microsomal membrane
Biochim Biophys Acta.
.
Abstract
Microsomal vesicles were centrifuged through sucrose density gradients containing deoxycholate. With 0.15% detergent electron transport enzymes and phosphatases could be separated. Increasing the deoxycholate concentration to 0.19% resulted in separation of the microsomal material into five bands containing (in order from the top of the gradient) adenosine monophosphatase, inosine diphosphatase and some glucose-6-phosphatase (band 1); NADH-linked (band 2) and NADH-linked (band 3) electron transport enzymes; and glucose-6-phosphatase (bands 4 and 5). It appears that enzymes are arranged in specialized patches in the microsomal membrane.
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