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. 2006 May 10;11(5):334-44.
doi: 10.3390/11050334.

An improved NMR study of liposomes using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine as model

Oscar Cruciani  1 , Luisa Mannina, Anatoli P Sobolev, Cesare Cametti, AnnaLaura Segre
Affiliations

An improved NMR study of liposomes using 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine as model

Oscar Cruciani et al. Molecules. .

Abstract

In this paper we report a comparative characterization of Small Unilamellar Vesicles (SUVs), Large Unilamellar Vesicles (LUVs) and Multilamellar Vesicles (MLVs) prepared from 1-palmitoyl-2-oleoyl-sn-glycero-3-phospatidylcholine (POPC), carried out using two NMR techniques, namely High Resolution NMR in solution and High Resolution-Magic Angle Spinning (HR-MAS). The size and size distributions of these vesicles were investigated using the dynamic light scattering technique. An improved assignment of the (1)H-NMR spectrum of MLVs is also reported.

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Figures

Figure 1
Figure 1
POPC.
Figure 2
Figure 2
CONTIN analysis of the autocorrelation function for the sample of POPC LUVs: a monomodal distribution with an average hydrodynamic diameter of 145 ± 6 nm is obtained.
Figure 3
Figure 3
600 MHz 1H-NMR spectra of different classes of POPC (50mg/mL) liposomes: a) HR-MAS spectrum of MLVs, 12kHz at 314.8 K; b) HR-MAS spectrum of LUVs, 12kHz at 314.8 K; c) 1H NMR spectrum of SUVs, no spinning at 320.0K. The arrows indicate the chemical shift position of CH2-3.
Figure 4
Figure 4
1H-1H COSY map of POPC MLVs obtained using a spinning rate of 6 kHz. The calibrated temperature value of 303.9 K was obtained setting the temperature at 300.0 K.
See this image and copyright information in PMC

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