[Solubilization of components of outer membrane of Leptospira interrogans serovar mozdok by sodium desoxycholate and triton X-100 and evaluation of their immunogenic capacity and homologous and heterologous protection induced in hamsters]

Abstract

Two detergents (triton X-100 and sodium desoxycholate) were evaluated as solubilizing agents of the components of the outer membrane of Leptospira Pomona serovar mozdok. It was obtained a greater yielding of proteins on applying the treatment with triton X-100. The variants formulated starting from the extracts obtained showed a high immunogenicity and capacity of homologous protection against the challenge in hamster.