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. 2007 Dec;4(12):1019-21.
doi: 10.1038/nmeth1118. Epub 2007 Nov 4.

In situ proteolysis for protein crystallization and structure determination

Aiping Dong  1 , Xiaohui Xu, Aled M Edwards; Midwest Center for Structural Genomics; Structural Genomics Consortium; Changsoo Chang, Maksymilian Chruszcz, Marianne Cuff, Marcin Cymborowski, Rosa Di Leo, Olga Egorova, Elena Evdokimova, Ekaterina Filippova, Jun Gu, Jennifer Guthrie, Alexandr Ignatchenko, Andrzej Joachimiak, Natalie Klostermann, Youngchang Kim, Yuri Korniyenko, Wladek Minor, Qiuni Que, Alexei Savchenko, Tatiana Skarina, Kemin Tan, Alexander Yakunin, Adelinda Yee, Veronica Yim, Rongguang Zhang, Hong Zheng, Masato Akutsu, Cheryl Arrowsmith, George V Avvakumov, Alexey Bochkarev, Lars-Göran Dahlgren, Sirano Dhe-Paganon, Slav Dimov, Ludmila Dombrovski, Patrick Finerty Jr, Susanne Flodin, Alex Flores, Susanne Gräslund, Martin Hammerström, Maria Dolores Herman, Bum-Soo Hong, Raymond Hui, Ida Johansson, Yongson Liu, Martina Nilsson, Lyudmila Nedyalkova, Pär Nordlund, Tomas Nyman, Jinrong Min, Hui Ouyang, Hee-won Park, Chao Qi, Wael Rabeh, Limin Shen, Yang Shen, Deepthi Sukumard, Wolfram Tempel, Yufeng Tong, Lionel Tresagues, Masoud Vedadi, John R Walker, Johan Weigelt, Martin Welin, Hong Wu, Ting Xiao, Hong Zeng, Haizhong Zhu
Affiliations

In situ proteolysis for protein crystallization and structure determination

Aiping Dong et al. Nat Methods. 2007 Dec.

Abstract

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

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