Molecular cytogenetics of Salminus fish (Characiformes) based on 5S and 18S rRNA genes hybridization, fluorochrome staining and C-banding

Abstract

Karyotype, mapping of nucleolar and 5S rRNA genes and distribution of constitutive heterochromatin supposedly AT-rich were characterized on two isolate populations of Salminus brasiliensis, the biggest characid fish, and three population of Salminus hilarii. The diploid number 2n=50 and the karyotype formulae (10M+20SM+20ST/A) were the same to Salminus species studied. The position of 18S rDNA cluster identified by FISH coincide with chromomycin A3 labeling (CMA+) in the long arm telomeric portion of sixth pair. Subtle differences for the disposal of the 5S rRNA gene in the chromosome of the Salminus are presented. The distribution of the constitutive heterochromatins and DA/DAPI+ bands are described.