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. 2008 Jan;46(1):331-3.
doi: 10.1128/JCM.01251-07. Epub 2007 Nov 7.

Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results

Pamela K Cassiday  1 , Lucia C Pawloski, Tejpratap Tiwari, Gary N Sanden, Patricia P Wilkins
Affiliations

Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results

Pamela K Cassiday et al. J Clin Microbiol. 2008 Jan.

Abstract

Diphtheria surveillance depends on the rapid and reliable recognition of the toxin gene in Corynebacterium diphtheriae. Real-time PCR is a rapid tool to confirm the presence of the diphtheria toxin gene (tox) in an isolate or specimen. We report that some toxigenic Corynebacterium ulcerans strains show atypical results in a real-time PCR for tox.

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FIG. 1.
FIG. 1.
Real-time PCR graph showing the detection of tox subunits A and B in typical and atypical strains of C. ulcerans.
See this image and copyright information in PMC

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