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Multicenter Study
. 2008 Feb 1;177(3):342-7.
doi: 10.1164/rccm.200708-1142OC. Epub 2007 Nov 7.

Chromosomal aneusomy in bronchial high-grade lesions is associated with invasive lung cancer

Steinn Jonsson  1 , Marileila Varella-Garcia, York E Miller, Holly J Wolf, Tim Byers, Sarah Braudrick, Porntip Kiatsimkul, Marina Lewis, Timothy C Kennedy, Robert L Keith, Johannes Bjornsson, Annette McWilliams, Stephen Lam, Fred R Hirsch, Wilbur A Franklin
Affiliations
Multicenter Study

Chromosomal aneusomy in bronchial high-grade lesions is associated with invasive lung cancer

Steinn Jonsson et al. Am J Respir Crit Care Med. .

Abstract

Rationale: The development of lung cancer (LC) is accompanied by field changes in the airway mucosa that may have prognostic importance.

Objectives: To compare patients with prevalent LC to control subjects regarding their histologic dysplasia scores and chromosomal aneusomy as measured by fluorescence in situ hybridization (FISH).

Methods: The most advanced bronchial histology lesion was assessed from each of 44 LC cases and 90 cancer-free control subjects using a four-color FISH probe set encompassing the chromosome 6 centromere, 5p15.2, 7p12 (epidermal growth factor receptor), and 8q24 v-myc myelocytomatosis viral oncogene homolog (MYC) sequences. Histology grades were coded as dysplasia (moderate or severe) or carcinoma in situ (CIS).

Measurements and main results: CIS was the highest histologic grade for 32 subjects, and dysplasia was the highest grade for 102 subjects (54 moderate, 48 severe). Chromosomal aneusomy was seen in 64% of the LC cases, but in only 31% of the control subjects (odds ratio [OR], 4.68; 95% confidence interval [CI]. 1.97-11.04). Among those with any level of dysplasia, the OR for positive FISH and LC was 2.28 (95% CI, 0.75-6.86). Among those with CIS, the OR for positive FISH and LC was 5.84 (95% CI, 1.31-26.01).

Conclusions: Chromosomal aneusomy is associated with LC. Prospective examination of aneusomy as a precursor lesion that predicts LC is needed.

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Figures

<b>Figure 1.</b>
Figure 1.
Bronchial epithelium area selected for fluorescence fluorescence in situ hybridization (FISH) analysis. (A) Area with carcinoma in situ stained by hematoxylin and eosin. Magnification: ×20. (B) The same area in the sequential slide submitted to the LAVysion FISH assay stained by 4′,6-diamidino-2-phenylindole (DAPI). Magnification: ×40. The area included in the open rectangle is shown at 100× magnification for combination of fluors in (C) DAPI and SpectrumRed, (D) DAPI and SpectrumGreen, (E) DAPI and SpectrumGold, and (F) DAPI and SpectrumAqua. The specimen displayed aneusomy for (C) epidermal growth factor receptor, (D) 5p15.2, (E) v-myc myelocytomatosis viral oncogene homolog (MYC), and (F) Chromosome Enumeration Probe 6 (CEP6) regions. Arrows indicate single nuclei with extra copies of the tested target DNA.
<b>Figure 2.</b>
Figure 2.
Frequency of moderate dysplasia, severe dysplasia, and carcinoma in situ (CIS) among cases and control subjects.
See this image and copyright information in PMC

References

    1. Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics, 2007. CA Cancer J Clin 2007;57:43–66. - PubMed
    1. Peto R, Lopez AD, Boreham J, Thun M, Heath C, Doll R. Mortality from smoking worldwide. Br Med Bull 1996;52:12–21. - PubMed
    1. Jonsson S, Thorsteinsdottir U, Gudbjartsson DF, Jonsson HH, Kristjansson K, Arnason S, Gudnason V, Isaksson HJ, Hallgrimsson J, Gulcher JR, et al. Familial risk of lung carcinoma in the Icelandic population. JAMA 2004;292:2977–2983. - PubMed
    1. Bailey-Wilson JE, Amos CI, Pinney SM, Petersen GM, de Andrade M, Wiest JS, Fain P, Schwartz AG, You M, Franklin W, et al. A major lung cancer locus maps to chromosome 6q23-25. Am J Hum Genet 2004;75:460–474. - PMC - PubMed
    1. Hirsch FR, Franklin WA, Gazdar AF, Bunn PA. Early detection of lung cancer: clinical perspectives of recent advances in biology and radiology. Clin Cancer Res 2001;7:5–22. - PubMed

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