doi: 10.1038/nmeth1132.
Epub 2007 Nov 11.
An FKBP destabilization domain modulates protein levels in Plasmodium falciparum
Affiliations
- PMID: 17994030
- DOI: 10.1038/nmeth1132
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An FKBP destabilization domain modulates protein levels in Plasmodium falciparum
Nat Methods.
2007 Dec.
Abstract
To enhance the repertoire of molecular tools for studying malaria parasite biology, we adapted a ligand-regulatable FKBP protein destabilization domain (ddFKBP) for use in P. falciparum. We destabilized the reporter yellow fluorescent protein (YFP) and the P. falciparum protease falcipain-2 in a ligand-reversible manner by tagging with ddFKBP. The swollen food vacuole phenotype of falcipain-2 knockout parasites could be rescued in a Shld1 ligand-dependent fashion by falcipain-2-ddFKBP expression.
Comment in
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Switching parasite proteins on and off.Nat Methods. 2007 Dec;4(12):999-1000. doi: 10.1038/nmeth1207-999. Nat Methods. 2007. PMID: 18049467 No abstract available.
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