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. 2007 Dec;4(12):1007-9.
doi: 10.1038/nmeth1132. Epub 2007 Nov 11.

An FKBP destabilization domain modulates protein levels in Plasmodium falciparum

Christopher M Armstrong  1 , Daniel E Goldberg
Affiliations

An FKBP destabilization domain modulates protein levels in Plasmodium falciparum

Christopher M Armstrong et al. Nat Methods. 2007 Dec.

Abstract

To enhance the repertoire of molecular tools for studying malaria parasite biology, we adapted a ligand-regulatable FKBP protein destabilization domain (ddFKBP) for use in P. falciparum. We destabilized the reporter yellow fluorescent protein (YFP) and the P. falciparum protease falcipain-2 in a ligand-reversible manner by tagging with ddFKBP. The swollen food vacuole phenotype of falcipain-2 knockout parasites could be rescued in a Shld1 ligand-dependent fashion by falcipain-2-ddFKBP expression.

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