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. 1991 Oct;10(5):495-501.
doi: 10.1007/BF01025477.

Recombinant human hemoglobin: expression and refolding of beta-globin from Escherichia coli

C Fronticelli  1 , J K O'Donnell, W S Brinigar
Affiliations

Recombinant human hemoglobin: expression and refolding of beta-globin from Escherichia coli

C Fronticelli et al. J Protein Chem. 1991 Oct.

Abstract

A plasmid analogous to the one described by Nagai and Thogersen (Nature, 309, 810-812, 1984) has been constructed for the expression of globins in E. coli. Induction with nalidixic acid produces high yields of a fusion protein, NS1-FX-beta-globin, where NS1 represents 81 residues of a flu virus protein and FX represents a blood-clotting Factor Xa recognition sequence, Ile-Glu-Gly-Arg. This fusion protein is readily solubilized in 50 mM NaOH and remains in solution when the pH is adjusted to 8.6. Under these conditions, the fusion protein is hydrolyzed by activated Factor X, giving authentic beta-globin which can be folded in the presence of cyanohemin and native alpha-chains to produce a tetrameric hemoglobin with the functional properties of natural human hemoglobin.

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