Quantitative aberrations of hypermethylated RASSF1A gene sequences in maternal plasma in pre-eclampsia
- PMID: 17994635
- DOI: 10.1002/pd.1897
Quantitative aberrations of hypermethylated RASSF1A gene sequences in maternal plasma in pre-eclampsia
Abstract
Objective: To study if quantitative aberrations in circulating placental-derived hypermethylated RASSF1A DNA in maternal plasma are associated with pre-eclamptic pregnancies.
Method: Maternal plasma and placental tissues from third-trimester pre-eclamptic women and gestational-age matched normotensive controls were studied. Real-time PCR was performed to quantify RASSF1A concentrations before and after methylation-sensitive restriction digestion in a duplex assay, where ss-actin concentrations were quantified as an internal control to confirm complete enzyme digestion.
Results: The median concentrations of hypermethylated RASSF1A were 4.3-fold higher in maternal plasma of pre-eclamptic subjects than in controls. There was no significant difference between the extent of RASSF1A hypermethylation in placental tissues obtained from pre-eclamptic and control pregnancies.
Conclusion: This study demonstrated the potential utility of hypermethylated RASSF1A sequences in maternal plasma as a gender- and polymorphism-independent marker for pre-eclampsia.
Copyright (c) 2007 John Wiley & Sons, Ltd.
Similar articles
-
[Quantitative detection of the hypermethylated RASSF1A gene in maternal plasma of pre-eclampsia].Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Feb;27(1):73-6. doi: 10.3760/cma.j.issn.1003-9406.2010.01.016. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010. PMID: 20140873 Chinese.
-
[Predictive value of placenta-derived RASSF1A sequence expression in maternal plasma for pre-eclampsia].Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014 Feb;31(1):25-8. doi: 10.3760/cma.j.issn.1003-9406.2014.01.006. Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2014. PMID: 24510557 Chinese.
-
Association of fetal-derived hypermethylated RASSF1A concentration in placenta-mediated pregnancy complications.Placenta. 2013 Jan;34(1):57-61. doi: 10.1016/j.placenta.2012.11.001. Epub 2012 Nov 24. Placenta. 2013. PMID: 23187089
-
Cell-free nucleic acids as potential markers for preeclampsia.Placenta. 2011 Feb;32 Suppl:S17-20. doi: 10.1016/j.placenta.2010.06.018. Placenta. 2011. PMID: 21257079 Review.
-
Placental gene expression and pre-eclampsia.Placenta. 2008 Mar;29 Suppl A:S78-82. doi: 10.1016/j.placenta.2007.09.005. Epub 2007 Oct 23. Placenta. 2008. PMID: 17959244 Review.
Cited by
-
Extracellular nucleic acids in maternal circulation as potential biomarkers for placental insufficiency.DNA Cell Biol. 2012 Jul;31(7):1221-32. doi: 10.1089/dna.2011.1530. Epub 2012 Feb 24. DNA Cell Biol. 2012. PMID: 22364204 Free PMC article. Review.
-
Dynamic Changes of Fetal-Derived Hypermethylated RASSF1A and Septin 9 Sequences in Maternal Plasma.Reprod Sci. 2021 Apr;28(4):1194-1199. doi: 10.1007/s43032-020-00416-y. Epub 2021 Jan 8. Reprod Sci. 2021. PMID: 33417202
-
Endocrine disruptors, environmental oxygen, epigenetics and pregnancy.Front Biosci (Elite Ed). 2011 Jan 1;3(2):690-700. doi: 10.2741/e279. Front Biosci (Elite Ed). 2011. PMID: 21196344 Free PMC article. Review.
-
Impact of DNA methylation on trophoblast function.Clin Epigenetics. 2011 Nov 1;3(1):7. doi: 10.1186/1868-7083-3-7. Clin Epigenetics. 2011. PMID: 22414254 Free PMC article.
-
Systematic identification of placental epigenetic signatures for the noninvasive prenatal detection of Edwards syndrome.PLoS One. 2010 Nov 30;5(11):e15069. doi: 10.1371/journal.pone.0015069. PLoS One. 2010. PMID: 21152411 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical