Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Nov 1;63(Pt 11):983-6.
doi: 10.1107/S1744309107051524. Epub 2007 Oct 26.

Crystallization and preliminary X-ray diffraction studies of tetrameric malate dehydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1

Tomomi Fujii  1 , Tadao Oikawa, Ikuo Muraoka, Kenji Soda, Yasuo Hata
Affiliations

Crystallization and preliminary X-ray diffraction studies of tetrameric malate dehydrogenase from the novel Antarctic psychrophile Flavobacterium frigidimaris KUC-1

Tomomi Fujii et al. Acta Crystallogr Sect F Struct Biol Cryst Commun. .

Abstract

Flavobacterium frigidimaris KUC-1 is a novel psychrotolerant bacterium isolated from Antarctic seawater. Malate dehydrogenase (MDH) is an essential metabolic enzyme in the citric acid cycle and has been cloned, overexpressed and purified from F. frigidimaris KUC-1. In contrast to the already known dimeric form of MDH from the psychrophile Aquaspirillium arcticum, F. frigidimaris MDH exists as a tetramer. It was crystallized at 288 K by the hanging-drop vapour-diffusion method using ammonium sulfate as the precipitating agent. The crystal diffracted to a maximum resolution of 1.80 A. It contains one tetrameric molecule in the asymmetric unit.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Crystal of psychrophilic MDH from F. frigidimaris KUC-1 obtained at 277 K. The dimensions of the crystal were 0.70 × 0.10 × 0.02 mm.
Figure 2
Figure 2
Crystals of psychrophilic MDH from F. frigidimaris KUC-1 obtained at 288 K. The dimensions of the largest crystal were 1.0 × 0.15 × 0.05 mm.
See this image and copyright information in PMC

References

    1. Bradford, M. M. (1976). Anal. Biochem.72, 248–254. - PubMed
    1. Breuil, C. & Kushner, D. J. (1975). Can. J. Microbiol.21, 423–433. - PubMed
    1. Bruni, V., Gugliandolo, C., Maugeri, T. & Allegra, A. (1999). New Microbiol.22, 357–363. - PubMed
    1. Collaborative Computational Project, Number 4 (1994). Acta Cryst. D50, 760–763. - PubMed
    1. Dalhus, B., Saarinen, M., Sauer, U. H., Eklund, P., Johansson, K., Karlsson, A., Ramaswamy, S., Bjørk, A., Synstad, B., Naterstad, K., Sirevåg, R. & Eklund, H. (2002). J. Mol. Biol.318, 707–721. - PubMed

Publication types

Substances

LinkOut - more resources