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. 1991 Oct;83(10):901-4.

The production of a bispecific anti-CEA, anti-hapten (4-amino-phthalate) hybrid-hybridoma

F Lloyd Jr  1 , M Goldrosen
Affiliations

The production of a bispecific anti-CEA, anti-hapten (4-amino-phthalate) hybrid-hybridoma

F Lloyd Jr et al. J Natl Med Assoc. 1991 Oct.

Abstract

A standard hybridoma fusion technique was used to produce a monoclonal antibody capable of binding both carcinoembryonic antigen (CEA) and the hapten 4-amino-phthalate. A hypoxanthine-aminopterin-thymidine (HAT) sensitive anti-CEA hybridoma and KLH-phthalate immunized spleen cells were hybridized to yield clones producing bispecific monoclonal antibodies. The desired bispecific antibody was identified using both enzyme-linked immunosorbent assay (ELISA) and radio-immunoassay. The resultant hybrid-hybridoma or "tridoma" was subcloned and expanded to yield a stable population. Bifunctional antibody was then isolated from the various possible recombinants by ion exchange chromatography. This general method may be used to produce bispecific monoclonals against a wide variety of tumor associative antigens and reagents for immunodetection or treatment.

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