Selective translation of mRNAs in the left ventricular myocardium of the mouse in response to acute pressure overload
- PMID: 18036610
- PMCID: PMC2246077
- DOI: 10.1016/j.yjmcc.2007.10.011
Selective translation of mRNAs in the left ventricular myocardium of the mouse in response to acute pressure overload
Abstract
During pressure overload hypertrophy, selective changes in cardiac gene expression occur that regulate growth and modify the structural and functional properties of the myocardium. To determine the role of translational mechanisms, a murine model of transverse aortic constriction was used to screen a set of specified mRNAs for changes in translational activity by measuring incorporation into polysomes in response to acute pressure overload. Candidate mRNAs were selected on the basis of two main criteria: (1) the 5'-untranslated region of the mRNA contains an excessive amount of secondary structure (DeltaG<-50 kCal/mol), which is postulated to regulate efficiency of translation, and (2) the protein product has been implicated in the regulation of cardiac hypertrophy. After 24 h of transverse aortic constriction, homogenates derived from the left ventricle were layered onto 15-50% linear sucrose gradients and resolved into monosome fractions (messenger ribonucleoprotein particles) and polysome fractions by density gradient ultracentrifugation. The levels of mRNA in each fraction were quantified by real-time RT-PCR. The screen revealed that pressure overload increased translational activity of 6 candidate mRNAs as determined by a significant increase in the percentage of total mRNA incorporated into the polysome fractions. The mRNAs code for several functional classes of proteins linked to cardiac hypertrophy: the transcription factors c-myc, c-jun and MEF2D, growth factors VEGF and FGF-2 and the E3 ubiquitin ligase MDM2. These studies demonstrate that acute pressure overload alters cardiac gene expression by mechanisms that selectively regulate translational activity of specific mRNAs.
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References
-
- Berenji K, Drazner MH, Rothermel BA, Hill JA. Does load-induced ventricular hypertrophy progress to systolic heart failure? Am J Physiol Heart Circ Physiol. 2005 Jul;289(1):H8–H16. - PubMed
-
- Cooper G., IV Cardiocyte adaptation to chronically altered load. Annu Rev Physiol. 1987;49:501–18. - PubMed
-
- Marino TA, Kent RL, Uboh CE, Fernandez E, Thompson EW, Cooper G., IV Structural analysis of pressure versus volume overload hypertrophy of cat right ventricle. Am J Physiol. 1985 Aug;249(2 Pt 2):H371–9. - PubMed
-
- Swynghedauw B. Phenotypic plasticity of adult myocardium: molecular mechanisms. J Exp Biol. 2006 Jun;209(Pt 12):2320–7. - PubMed
-
- Hannan RD, Jenkins A, Jenkins AK, Brandenburger Y. Cardiac hypertrophy: a matter of translation. Clin Exp Pharmacol Physiol. 2003 Aug;30(8):517–27. - PubMed
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