[Effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb]

Abstract

The effect of phytohormones on plant regeneration in callus culture of Iris ensata Thunb. was studied. 2,4-Dichlorophenoxyacetic acid (2,4-D) proved to be the most convenient auxin for the induction and subculturing of morphogenic callus. Biogenic calluses were induced from isolated embryos at the wax-ripeness stage in MS medium supplemented with 2,4-D (1.0-2.0 mg/l) and kinetin (0.2-0.5 mg/l). Changes in the medium hormonal composition favor the development of adventitious structures. The setting of vegetative bugs took place in the presence of 6-benzylaminopurine, while the development of shoots and root primordia was observed after 2,4-D replacement with indoleacetic acid (2.0 mg/l). The root initiation in regenerating plants required a hormone-free medium. The properties of seedling growth from native regenerating I. ensata were studied. Analysis of the shoot structure demonstrated that in vitro cultured plants are at the juvenile stage.