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Comparative Study
. 1991;77(8):697-702.
doi: 10.1007/BF00928685.

Application of the western blotting procedure for the immunodiagnosis of human toxocariasis

J F Magnaval  1 , R Fabre, P Maurières, J P Charlet, B de Larrard
Affiliations
Comparative Study

Application of the western blotting procedure for the immunodiagnosis of human toxocariasis

J F Magnaval et al. Parasitol Res. 1991.

Abstract

To improve the immunodiagnosis of human toxocaral disease, a sensitive and specific assay using the Western blotting procedure (WB) with excretory-secretory antigens from Toxocara canis larvae (TES) was developed and compared with the standard enzyme-linked immunosorbent assay method (TES-ELISA) using the same antigens. We tested groups of sera from laboratory animals or patients presenting with toxocariasis or other helminthic diseases and a group of sera from people dwelling in an area endemic for toxocariasis who exhibited hypereosinophilia. Statistically, the WB assay correlated well with TES-ELISA, but the former was more specific for banding patterns corresponding to low-molecular-weight fractions, thus avoiding problems of cross-reactivity with sera infected with other helminthic diseases.

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