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Review
. 2007 Dec;145(4):1129-43.
doi: 10.1104/pp.107.106690.

Chloroplast vector systems for biotechnology applications

Dheeraj Verma  1 , Henry Daniell
Affiliations
Review

Chloroplast vector systems for biotechnology applications

Dheeraj Verma et al. Plant Physiol. 2007 Dec.
No abstract available

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
Schematic representation of the chloroplast-specific expression cassette. Map of the chloroplast expression vector shows the integration sites, promoters, selectable marker genes, regulatory elements, and genes of interest. For a list of regulatory elements and genes of interest used for chloroplast transformation, refer to Table II.
Figure 2.
Figure 2.
Selection of transplastomic plants. Shown are representative photographs of transplastomic tobacco and lettuce shoots undergoing first (A and D), second (B and E), and third (C and F, rooting) rounds of selection, respectively.
Figure 3.
Figure 3.
Evaluation of transgene integration into the chloroplast genome. DNA isolated from putative transplastomic shoots are analyzed by PCR and Southern-blot analysis. A, 3P/3M and 5P/2M primer pairs (Kumar and Daniell, 2004) are used for PCR analysis; PCR products of 3P/3M primers. Lane 1, Untransformed plant; lanes 2 to 4, transformed lines (1.6 kb); lane 1kb+, DNA marker; lanes 5 to 7, PCR product with 5P/2M primers (3.2 kb) in transformed lines. B, The chloroplast genome is probed with a radiolabeled flank fragment. Lane 1, Untransformed plant; lanes 2 and 4, homoplasmic transplastomic plant; lane 3, heteroplasmic transplastomic plant.
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