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Review
. 2007:24:297-309.
doi: 10.1080/02648725.2007.10648105.

Genetic modification of human embryonic stem cells

Xiaofeng Xia  1 , Su-Chun Zhang
Affiliations
Review

Genetic modification of human embryonic stem cells

Xiaofeng Xia et al. Biotechnol Genet Eng Rev. 2007.
No abstract available

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Figures

Figure 1
Figure 1. Location of transfected cells in a hES colony
Cells were transfected with a GFP construct using FuGene6 following manufacturer’s instruction. Most of the transfected green cells were located on the edge of the colony.
Figure 2
Figure 2. Strategy to achieve tissue specific gene slicing in hES cells and derivatives
A, structures of the two constructs used in the strategy. One construct drives the expression of cre recombinase under a tissue specific promoter. The other construct uses U6 promoter to drive the expression of small hairpin RNA. The two reverse complementary double strand oligos were separated by a floxed stop codon. B, In the specific tissue where the promoter is active, cre recombinase is expressed so that the floxed stop codon is removed through cre/loxp recombination. This enables the transcription of the full self-complementary oligo so that small hairpin RNAs are generated which lead to knockdown of the target gene.
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