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. 2007 Dec 1;32(23):3408-10.
doi: 10.1364/ol.32.003408.

Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

Dylan M Owen  1 , Egidijus Auksorius, Hugh B Manning, Clifford B Talbot, Pieter A A de Beule, Christopher Dunsby, Mark A A Neil, Paul M W French
Affiliations

Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

Dylan M Owen et al. Opt Lett. .

Abstract

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.

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