Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Oct;47(5):928-31.

[Production of monoclonal antibodies to Cymbidium mosaic virus and application in orchids virus detection]

[Article in Chinese]
Chun-Mei Meng  1 , Jian-Xiang Wu, Li Xie, Jin-Kai Zheng, Jian Hong
Affiliations
  • PMID: 18062276

[Production of monoclonal antibodies to Cymbidium mosaic virus and application in orchids virus detection]

[Article in Chinese]
Chun-Mei Meng et al. Wei Sheng Wu Xue Bao. 2007 Oct.

Abstract

Three hybridoma cell lines, 2C6, 5B7 and 12G9, secreting monoclonal antibodies (McAbs) against Cymbidium mosaic virus (CymMV) were produced by fusing mouse myeloma cells (SP2/0) with spleen cells from BALB/ C immunized by the CymMV particles. The three McAbs could specifically react with CymMV. The titres of ascitic fluids of two McAbs are up to 10(-6) in I-ELISA. Isotypes and subclasses of the the three McAbs belong to IgG1. Isotypes of light strains of the three McAbs all belong to kappa. They were used in antigen-coated plate (ACP)-ELISA for CymMV detection, and ACP-ELISA could successfully detect 0.487 ng of purified CymMV or virus in plant sap diluted 1:10240. The presence of CymMV in field Orchids tissues was investigated with ACP-ELISA.

PubMed Disclaimer

Similar articles

See all similar articles

Publication types

LinkOut - more resources